Abstract: Aiming at the problems of the lack of online popular science resources and the extreme imbalance of educational resources in the promotion and popularization of traditional Chinese medicine culture, this paper analyzes the data related to the construction of online resources of traditional Chinese medicine with CiteSpace and content analysis, forming the idea of the construction of online popular science resources of traditional Chinese medicine culture. It further discusses the use of theoretical knowledge and concepts of traditional Chinese medicine to guide life and practice, and the cross integration of traditional Chinese medicine cultural knowledge and existing disciplines. Finally, it analyzed the use of animation, demonstration teaching, Chinese medicine website and other forms to build online popular science resources of Chinese medicine culture, in order to truly integrate Chinese medicine culture into people’s daily life and study, and to provide certain data support and theoretical guidance for promoting and inheriting Chinese medicine culture and promoting Chinese medicine to enter schools, communities, villages and families.