Abstract: Mobile digital museum has broken some limitations of traditional physical museum in terms of region, time, space and collection requirements, and it has the special advantages that can't be achieved by physical museum in the cultural dissemination of Anhui grand canal culture. In the development process of mobile digital museum, it is necessary to attach equal importance to the protection and propaganda of Anhuigrand canal culture, pay attention to the systematization of content in mobile digital museum and the characteristics of Anhui grand canal culture, and make sure that the system function modules of interface and content are restored, protected, inherited and designed on the basis of guaranteeing the authenticity of Anhui grand canal culture.