Abstract: The spatial structure of 100 demonstration sites of provincial leisure agriculture and rural tourism in Anhui Province was qualitatively depicted and quantitatively measured by ArcGIS, nearest neighbor distance index and compactness index. Results show that the demonstration sites of leisure agriculture and rural tourism in Anhui Province are widely distributed and have some differences. They are relatively dense in the south and the middle, and sparse in the north. Moreover, the overall spatial agglomeration degree of the demonstration sites is low and the distribution is relatively uniform. In addition, the regional form is compact and the regional accessibility is good, which is convenient for the flow of passengers and logistics. Therefore, the southern Anhui should take advantage of good regional tourism development conditions to create leisure agriculture and rural tourism brands; the central Anhui should rely on the source of tourists in Hefei Economic Circle, take the path of boutique and refinement; and the northern Anhui should dig deep into “production-life-ecology” resource to promote the further integration of agriculture and tourism.