Abstract: How to build a practical education system to meet the needs of entrepreneurship education is an urgent task for innovation and entrepreneurship education in colleges and universities. The goal of this study is to build a hierarchical entrepreneurial practice education system based on "consciousness-ability-practice", build a comprehensive training platform to enhance entrepreneurial awareness, enhance entrepreneurial ability and guide entrepreneurial practice, put forward a hierarchical training target system for entrepreneurial talents, integrate the key elements of entrepreneurial practice, establish an entrepreneurial practice education system to meet diversified and personalized needs, and explore the "four linkage" mechanism with curriculum, project, platform and park as the carrier based on the goal of entrepreneurial practice education and the "three levels" of innovation practice education, so as to improve the entrepreneurial practice education system in colleges and universities.