Abstract: The location entropy index and dynamic factor analysis were used to measure the high-tech industry agglomeration, industrial structure upgrading and green development level in the Yangtze River Economic Belt, and the relevant model of spatial measurement was used to analyze the impact of high-tech industry agglomeration on green development in the Yangtze River Economic Belt based on spatial perspective. It is found that there are inter-regional differences in the distribution of high-tech industry agglomeration in the Yangtze River Economic Belt, and the degree of high-tech industry agglomeration is higher in the eastern region; the green development level in the eastern region of the Yangtze River Economic Belt is higher than that in the central and western regions; the green development level in the Yangtze River Economic Belt has a strong spatial correlation, and the impact of high-tech industry agglomeration on green development has a certain spatial effect.