Abstract: Disney animated films in the 21st century violates the conventional filming models, forsakes the unchanged featuring of characters and challenges the social rules and stereotypes, which consequently reestablishes a new identity for queers, the social subordinate or outcast. The final acceptance in the films reflects the self-identification of queers in the reality. Based on culture studies, queer theories and anatomy of Disney animated films, this paper takes examples of several animations to attain a comprehensive analysis of the images of queers established by the mass media. This research will be helpful in the further study on the gender roles in mass media.