Abstract: Matlab is an important professional optional course for undergraduate of Optoelectronic Information Science and Engineering, which focuses on training students’ basic abilities required for mathematical modeling, problem analysis, etc. In order to improve teaching quality, measures such as enriching teaching content, adding case-based teaching design and online and offline mixed teaching models as well as strengthening the practical application of theoretical knowledge are adopted. The ability of students to apply Matlab programming to solve practical problems has been cultivated, and their learning effects have been improved.