Abstract: The deep integration of short video with mobile Internet, big data and 5G technology has reconstructed the publicity and ideological work form and also profoundly affected the ecology of publicity and ideological work in colleges and universities. Grasping the influence of short video field on the publicity and ideological work and examining the development opportunities and realistic dilemmas of the work in colleges and universities under the boom of short video use are the inevitable logic of publicity and ideological work in the new era to adapt to the new media and new technology development. In order to realize the symbiosis between short video field and publicity and ideological work of colleges and universities, it is necessary to make concerted efforts from four dimensions of subject, object, mediator and ring body to promote the righteousness and innovation of publicity and ideological work of colleges and universities in short video field.