Abstract: The construction of “new engineering” is the strategic layout to promote the comprehensive reform of higher education and promote high-quality entrepreneurship and employment of university graduates. Under the guidance of the “new engineering” concept, the biotechnology major has established interdisciplinary curriculum system, built high-level industry-university-research cooperation education platform, reformed the theoretical teaching method and practical teaching hardware environment, designed a “three-in-one” evaluation system, strengthened the construction of teaching staff and department of biotechnology, and built a comprehensive quality control and guarantee system, which comprehensively improved the quality of talent training by the “government-industry-university-research-application” integrated talent training model.