Abstract: Chinese traditional gardens are the product of the extension of Chinese philosophical thinking and cultural connotation, which contains the unique national aesthetic spatial thinking applicable to the development of China’s museums. Therefore, from this perspective, the construction of museum exhibition space mobility is explored, which is conducive to inheriting and carrying forward the essence of traditional culture through museums. With the method of field research and interdisciplinary research methods such as architecture and landscape architecture, this paper makes a deep interpretation of the Six Dynasties Museum in Nanjing, and explores the design organization of space mobility from the perspectives of spatial layout, visual organization, spatial circulation and spatial sequence, this paper explores the application of "spatial fluidity" in the exhibition space of museums.