Abstract: In the persona conception of Macbeth and Lady Macbeth in the drama Macbeth, Shakespeare gave the presentation to the dual temperament,i.e.androgyny, composed by the physical sex and social gender instead of the gender feature each of them should have. Each of their gender identity is not decided by the subject of willpower but is formed by the action that is concerned with the continous gender performance within the norm of social gender, which reflects the discrimination and prejudice to female in the malepower-centered society characteristics of western tradition.